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אשרף בריק

אשרף בריק

פרופסור
972-4-8291795+
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קבוצת המחקר שלנו מפתחת גישות סינטטיות חדשות לסינטזה כימית של חלבונים הומוגניים בעלי מודיפיקציות שלאחר תרגום (לדוגמא, חלבונים שעברו יוביקווטינציהופוספורילציה) המשמשים עבור אנליזות מבניות, ביוכימיות, ביופיזיקליות ופונקציונליות.

א) סינטזה כימית וסמי-כימית של חלבונים בעלי מודיפיקציות שלאחר תרגום.
ב) חקירת סגנליהיוביקוויטין ודומיו בשיטות של כימיה ביולוגית.
ג) סינטזה כימית של היסטונים שעברו מודיפקציות.
ד) ויסות פעילות אנזימים על ידי שימוש במולקולות קטנות, פפטידים ופפטידומימטיקה

  1. Suman Kumar Maity#, Guy Mann#, Muhammad Jbara, Shay Laps, Guy Kamnesky, and Ashraf Brik; Palladium-Assisted Removal of a Solubilizing Tag from a Cys Side Chain To Facilitate Peptide and Protein Synthesis (# these authors contributed equally), Organic Letters, 2016, 18, 3026–3029, ACS Editor Choise.
  2. Roman Meledin, Sachitanand M. Mali, Sumeet Singh and Ashraf Brik; Protein ubiquitination via Dehydroalanine: Development and Insights into the Diastereoselective 1,4-Addition Step, Organic & Biomolecular Chemistry, 2016, 14, 4817 – 4823.
  3. Suman Kumar Maity#, Muhammad Jbara#, Shay Laps and Ashraf Brik; Efficient Palladium Assisted One-pot and Rapid Deprotection of Cysteine(Acetamidomethyl) Following Native Chemical Ligation and/or Desulfurization to Expedite Chemical Protein Synthesis (# these authors contributed equally), AngewandteChemie ,2016, 55, 1 – 6.
  4. Muhammad Jbara#, Suman Kumar Maity#, MallikantiSeenaiah, and Ashraf Brik, Palladium Mediated Rapid Deprotection of N-terminal Cysteine Under Native Chemical Ligation Conditions for the Efficient Preparation of Synthetically Challenging Proteins (# these authors contributed equally), Journal of American Chemical Society, 2016, 138 ,5069–5075.
  5. PushparathinamGopinath, ShimritOhayon, MickalNawatha and Ashraf Brik, Chemical and Semisynthetic Approaches to Study and Target Deubiquitinases, Chemical Soiciety Reviews, Advanced Article.
  6. Michael Morgan, Mahmood Haj-Yahya, Alison E. Ringel, PrasanthiBandi, Ashraf Brik and Cynthia Wolberger*, Structural Basis for Histone H2B deubiquitination by SAGA DUB Module,Science, 2016, 351 725-728.
  7. SomasekharBondalapati#, Muhammad Jbara# and Ashraf Brik*; Expanding the Chemical Toolbox for the Synthesis of Large and Uniquely Modified Proteins (# these authors contributed equally), Nature Chemistry, 2016, 8, 407–418.
  8. Muhammad Jbara, Suman Kumar Maity, Michael Morgan, Cynthia Wolberger* and Ashraf Brik*: Total Chemical Synthesis of Phosphorylated Histone H2A at Tyr57 Reveals Insight into the Inhibition Mode of the SAGA Deubiquitinating Module, AngewandteChemie ,2016,55, 4972-6.
  9. PushparathinamGopinath and Ashraf Brik*, 2,2'-Azobis[2-(2-imidazolin-2-yl)propane] Dihydrochloride, e-EROS, In press.
  10. Suman Kumar Maity, Muhammad Jbara and Ashraf Brik*: Chemical and Semisynthesis of Modified Histones, Journal of Peptide Science, first published online: 18 JAN 2016 (Festschrift for the journal on occasion of the 70th birthday of Stephen BH Kent).
  11. Roman Meledin, Sachitanand M. Mali and Ashraf Brik; Pushing the Boundaries of Chemical Protein Synthesis: The Case of Ubiquitin Chains, Polyubiquitinated Peptides and Proteins; The Chemical Record, 2016, 16, 509–519.

Full publications list

Name E-Mail
Dr. Guy Kamnesky, Lab manager guykam@technion.ac.il
Dr.Hao Sun (Postdoc) haosun@campus.technion.ac.il
Dr. Babu Ganga (Postdoc) gangababu777@gmail.com
Dr. Sathish Gandhesiri (Postdoc) satish.ga@campus.technion.ac.il
Roman Meledin (Postdoc) meledinroman@gmail.com
Sumeet Singh (Ph.D.) sumeet.ballia@gmail.com
 Muhammad Jbara (Ph.D.) Jbara@technion.ac.il
Mickal Abd Alhadi mickal.soso@gmail.com
Guy Mann guym@technion.ac.il
Emad Eid Semadeid_m@campus.technion.ac.il
Shay Laps shay.laps@campus.technion.ac.il
Muna Msallam smmunmun@campus.technion.ac.il
Maria Levinman levinman@campus.technion.ac.il
Gabor Boross (Postdoc) gboross@campus.technion.ac.il

 

Chemist.