Single-Molecule Spectroscopy and Imaging in Biological Systems

Physical and Analytical Chemistry Seminar

Lecturer: Shira Bar-Zvi

06-06 Nov 2018 @ 15:30

Location: Faculty Seminar Room


Single molecule spectroscopy and imaging techniques have evolved for the past 30 years, beyond the early low temperature studies in crystals, to include single molecules in solution, in polymers, as well as in whole cells and biological tissues. The merit of this approach is that by not requiring ensemble averaging, it yields a variety of physical effects that could only be observed for individual molecules; including observations of spectral diffusion, blinking and photoinduced recovery of emitters, which stimulated further development of photoswitchable fluorescent protein labels. Current techniques and methodologies which enable investigations of heterogeneous environments, will be presented, as well as analyzing distribution of protein structures and dynamics in cellular systems.


Moerner, W. E., Shechtman, Y. & Wang, Q. Single-molecule spectroscopy and imaging over the decades. Faraday Discuss. (2015).