seminars

A METHYL-TROSY APPROACH TO NMR STUDIES OF LARGE DNAS AND PROTEIN-DNA COMPLEXES

Physical and Analytical Chemistry Seminar

Lecturer: Dr. Gili Abramov Departments of Molecular Genetics, Biochemistry, and Chemistry, University of Toronto, Canada

Date:
22-22 Dec 2020 @ 15:30

Location: Join Zoom Meeting at https://technion.zoom.us/j/98640529463

A methyl-TROSY approach to NMR studies of large DNAs and protein-DNA complexes

High-molecular-weight complexes that contain both protein and nucleic acid components are involved in many of the important functions in the cell including transcription, packaging, and repair. Developments in solution NMR spectroscopy, specifically methyl-TROSY approaches, have facilitated the study of the protein portions of these complexes, while studies of the nucleic acids have proven challenging. A new approach for labeling DNA molecules with methyl-group probes at specific sites has been developed, followed by the use of optimized NMR methods to obtain high-quality data in studies of these large molecular complexes. The methodology is demonstrated on the 150 base-pairs DNA molecule of the nucleosome core particle, the fundamental building block of chromatin. DNA methylation is also an important epigenetic mark. In mammals, CpG methylation serves as a regulation mechanism for gene expression. Methylation of promoter regions has been associated with tumorous cells including cancer. The effect of CpG methylation on the structural dynamics of the nucleosome core particle is demonstrated.